Journal of Microbes and Infection, September 2009, Vol. 4, No. 3
Advances in mechanisms of bacterial resistance to linezolid and related detection methods 1. No. 263 Hospital of Chinese People s Liberation Army, Beijing 101149, China; 2. Dalian Medical University,Abstract: Linezolid is an antibacterial belonging to the oxazolidinone class of antibiotics. The importance of linezolid as an antibiotic is related to its activity against a number of clinically significant Gram-positive cocci, including
multidrug-resistant staphylococci and vancomycin-resistant enterococci. Although bacterial resistance to linezolid is not
a major issue at present, its development in clinical isolates should be paid more attention. In this article, the
mechanisms of bacterial resistance to linezolid, and related detection methods were reviewed. We showed that
bacterial resistance is mediated by a single-nucleotide mutation and the production of methyltransferase, however,
other potential mechanisms have not yet been determined. The detection methods for linezolid resistance include
microbiological and molecular biological methods, some of which would benefit from technological improvements. Key words: Linezolid; Resistance; Oxazolidinone; Bacteria
( methicillin-resistant Staphylococcus aureus, MRSA)
( vancomycin-resistant enterococci,
Corresponding author: XIA Meng-Y an, E-mail: Yixmy@ 126. com
Journal of Microbes and Infection, September 2009, Vol. 4, No. 3
( chloramphenicol-florfenicol resistance,
Journal of Microbes and Infection, September 2009, Vol. 4, No. 3
[ 1] Mutnick AH, Enne V, Jones RN. Linezolid resistance
since 2001: Sentry antimicrobial surveillance program
[ J] . Ann Pharmacother, 2003, 37( 6) : 769-774
dependence of emergence of resistance to linezolid in
Enterococcus faecalis in vivo[ J] . J Infect Dis, 2007, 195
[ 3] Potoski BA, Adams J, Clarke L, et al. Epidemiological
Staphylococci[ J] . Clin Infect Dis, 2006, 43( 3) : 165-171
[ 4] Hillemann D, R sch-Gerdes S, Richter E.
selected linezolid-resistant Mycobacterium mutants [ J] .
Antimicrob Agents Chemother, 2008, 52( 2) : 800-801
[ 5] Ruggero KA, Schroeder LK, Schreckenberger PC, et al.
Nosocomial superinfections due to linezolid-resistant
Enterococcus faecalis: evidence for a gene dosage effect
Journal of Microbes and Infection, September 2009, Vol. 4, No. 3
on linezolid MICs[ J] . Diagn Microbiol Infect Dis, 2003,
against Staphylococci and Enterrococci [ J ] .
[ 6] Tsiodras S, Gold HS, Sakoulas G, et al.
[ 11] Marshall SH, Donskey CJ, Hutton-Thomas R, et al.
resistance in a clinical isolate of Staphylococcus aureus
Gene dosage and linezolid resistance in Enterococcus
[ J] . Lancet, 2001, 358( 9277) : 207-208
faecium and Enterococcus faecalis[ J] . Antimicrob Agents
Chemother, 2002, 46( 10) : 3334-3336.
resistance in sequential Staphylococcus aureus isolated
[ 12] Qi C, Zheng X, Obtas A, et al. Comparision of testing
associated with a T2500A mutation in the 23S rRNA
methods for detection of decreased linezolid susceptibility
gene and loss of a single copy of rRNA [ J] . J Infect
due to G2576T mutation of the 23S rRNA gene in
Enterococcus faecium and Enterococcus faecalis[ J] . J
[ 8] Arias CA, Vallejo M, Reyes J, et al. Clinical and
microbiological aspects of linezolid resistance mediated
by the cfr gene encoding a 23S rRNA methyltransferase
[ J] . J Clin Microbiol, 2008, 46( 3) : 892-896
Enterococcus spp. by fluorescence in situ hybridization
[ 9] Elvira R, Sabine RG, Doris H. First linezolid-resistant
[ J] . J Clin Microbiol, 2007, 45( 10) : 3421-3423
clinical isolates of Mycobacterium tuberculosis [ J ] .
[ 14] Werner G, Strornmenger B, Klare I, et al. Molecular
Antimicrobial Agents Chemother, 2007, 51 ( 4 ) : 1534-
detection of linezolid resistance in Enterococcus faecium
and Enterococcus faecalis by use of 5 nuclease real-time
[ 10] Fred CT, Portia PW, Sheila S, et al. Accuracy of six
PCR compared to a modified classical approach[ J] . J
antimicrobial susceptibility methods for testing linezolid
Clin Microbiol, 2004, 42( 11) : 5327-5331
: http: / / www. ncbi. nlm. gov /Genbank /
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