D-91207 Lauf a. d. Pegnitz, Germany Personal Data
Project Management
Group Leader at the Department of Microbial Genetics (Head: Dr. Friedrich Götz, Tuebingen, Germany). Gene regulation in Staphylococci, development and application of inducible expression systems and further molecular biology tools for Staphylococci. Postdoctoral Research
Research Associate in the research group of Dr. F. Titgemeyer (Erlangen, Germany). Characterisation of a putative transcriptional Education
PhD thesis in the research group of Dr. W. Hillen (Erlangen, Germany). Title: “Gene Regulation in Bacillus subtilis via novel Integrative Elements and Tet Repressor Variants”. Degree: Dr. rer. nat. (“magna cum laude”). Research visit in the Section of Molecular Biology at the University of California at San Diego (USA). Accomplishment of in silico analyses of putative carbohydrate uptake systems of Streptomyces Diploma thesis in the research group of Dr. F. Titgemeyer (Erlangen, Germany). Title: “The Role of the Glucose Kinase GlkA in Carbon Regulation of Streptomyces coelicolor”. Earned Degree: Diplom-Biologe (“sehr gut“ = excellent). Laboratory Skills
DNA manipulation, e.g. cloning, sequencing, mobility shift assays; in vitro transposition and transposomics; yeast two hybrid set-ups; protein expression and purification; DNA sequence alignments; BLAST analyses (various servers); bioinformatics concerning e.g. protein-family classification. Escherichia coli, Bacillus subtilis, Staphylococcus aureus, Streptomyces coelicolor, Saccharomyces cerevisiae, Arabidopis Teaching Experience
Teaching of undergraduate students in lab courses. Supervision of diploma- and PhD students’ theses. Peer Reviewed Publications
Bertram, R., Kolb, M. and Hillen, W. (20__)
In vivo activation of Tetracycline repressor by Cre/lox mediated gene assembly” J Mol Microbiol Biotechnol, accepted
Resch, M., Roth, H. M., Kottmair, M., Sevvana, M., Bertram, R., Titgemeyer, F. and Muller
”Cloning, expression, purification, crystallization and preliminary X-ray diffraction
analysis of YvoA from Bacillus subtilis.
Acta Crystallogr Sect F Struct Biol Cryst Commun 65(Pt 4): 410-414

Leibig, M., Krismer, B., Kolb, M., Friede, A., Götz, F. and Bertram, R. (2008)
”Marker removal in staphylococci via Cre recombinase and different lox sites“
Appl Env Microbiol 74(5): 1316-1323
Bertram, R. and Hillen, W. (2008)
”The application of Tet repressor in prokaryotic gene regulation and expression”. Microb Biotechnol 1(1): 2-16
Kamionka, A., Majewski, M.; Roth, K., Bertram, R., Kraft, C. and Hillen, W. (2006)
”Induction of tetracycline repressor requires the binding of two inducers“. Nucleic Acids Res 34(14): 3834-3841 Köstner, M., Schmidt, B., Bertram, R. and Hillen, W. (2006)
”Generating tetracycline inducible auxotrophy in Escherichia coli and Salmonella enterica serovar Typhimurium by using an insertion element and a hyperactive transposase“. Appl Env Microbiol 72(7): 4717-4725

Bertram, R.
, Wünsche, A., Sprehe, M. and Hillen, W. (2006)
“Regulated Expression of HPrK/P does not affect Carbon Catabolite Repression of the xyn Operon and of rocG in Bacillus subtilis“. FEMS Microbiol Lett 259(1): 147-152

Bertram, R.
, Köstner, M. Müller, J., Vazquez Ramos, J. and Hillen, W. (2005)
”Integrative elements for Bacillus subtilis yielding tetracycline-dependent growth
phenotypes“. Nucleic Acids Res 33(18): e153

Henßler, E. M., Bertram, R., Wisshak, S. and Hillen, W. (2005)
”Tet repressor mutants with altered effector binding and allostery“. FEBS Journal
272(17): 4487-4496

Bertram, R.
, Kraft, C., Wisshak, S., Müller, J., Scholz, O. and Hillen, W. (2004)
”Phenotypes of combined Tet repressor mutants for effector and operator recognition
and allostery“. J Mol Microbiol Biotechnol 8(2): 104-110

Kamionka, A., Bertram, R. and Hillen, W. (2005)
”Tetracycline dependent conditional gene knockout in Bacillus subtilis“. Appl Env Microbiol 71(2): 728-733

Scholz, O., Henßler, E. M., Bail, J., Schubert, P., Bogdanska-Urbaniak, J., Sopp, S.,
Reich, M., Wisshak, S., Köstner, M., Bertram, R. and Hillen, W. (2004)
”Activity reversal of Tet repressor by single amino acid exchanges“.
Mol Microbiol 53(3): 777-789

Bertram, R.
, Schlicht, M., Mahr, K., Nothaft, H., Saier, J. M. jr. and Titgemeyer F. (2004)
In silico and transcriptional analysis of carbohydrate uptake systems of Streptomyces coelicolor A3(2)“. J Bacteriol 186(5): 1362-1373


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