Le métronidazole (Flagyl) reste la référence dans le traitement des infections anaérobies et des parasitoses comme la giardiase ou l’amibiase. Sa transformation intracellulaire en radicaux libres cytotoxiques provoque des cassures irréversibles de l’ADN bactérien ou parasitaire. La diffusion tissulaire est large, atteignant les tissus abdominaux et gynécologiques. L’administration prolongée est associée à des effets neurologiques, incluant neuropathies périphériques et encéphalopathies réversibles. L’association avec l’alcool déclenche une réaction de type antabuse. Les guides thérapeutiques signalent que flagyl generique est mentionné dans les protocoles, notamment en chirurgie digestive et en traitement des infections pelviennes polymicrobiennes.
Gmo.hr
Federal Ministry of Health and Women - Division IV/B/12 Radetzkystraße 2 A - 1030 Wien November 2003 NOTIFICATION of work with GMO large scale (tick where applicable) Classification of the intended work (tick where applicable)
Biosafety level 1 Biosafety level 2 Classification of GMO (tick where applicable)
bacteria other eucaryotic cells agricultural crops other plants other microorganisms farm animals human cell tissue other animals Work with GMM: culture volume: 500 ml - 1 l more than 600 liter ( l) (declare volume) minutes of the BSC attached: Informations on the carrier, BSC, BSO, project manager and premises: carrier: Company A address: Street B 1 A-1020 Vienna phone: 123456 fax: e-mail: office@companyA.at biological safety officer (BSO): phone: 123456 fax: e-mail: Dr.B@companyA,at qualification and education:
Ph.D. in Genetics, University of Vienna 3 years postdoc, University of Graz 5 years project manager Comp. D for more details see the attached curriculum vitae 1.2.1. deputy of the BSO: name: phone: 123456 fax: e-mail: Dr.C@companyA.at qualification and education:
Ph. D. in Biochemistry and Biotechnology; University postdoc, University for more details see the attached curriculum vitae members of the biological safety committee (BSC): 1.3.1. number: overall: 4 internal: 3 external: 1 1.3.2. internal members: name: qualification and education: see 1.2 qualification and education: see 1.2.1 qualification and education:
Ph. D. in Microbiology, University of Texas 10 years Biosafety Officer of Comp. G for more details see the attached curriculum vitae qualification and education:
qualification and education:
1.3.3. external members: name: Prof. Dr. E address: Inst. of Microbiology, University of Vienna, A-1030 phone: 112233 fax: e-mail: Dr.E@mibi.univie.ac.at qualification, education and present occupation: Ph.D. Biotechnology, University since 10 years , a. o. Prof. University of Vienna for more details see the attached curriculum vitae address: qualification, education and present occupation:
project manager (not applicable for work with GMO in biosafety level 1): phone: 123456 fax: e-mail: Dr.F@companyA.at qualification and education:
Ph D. in Genetics, University of Vienna postdoc, University for more details see the attached curriculum vitae information on the premises: 1.5.1. adress of the premises: Street B 1, A-1020 Vienna 1.5.3. description of the parts of the premises, relevant for the work with GMO and for safety (including attached construction plans, as well as description of the equipment according to Annex II of the ordinance on work with GMO in the contained use; BGBl. II Nr. 431/2002):
Room Nr. 1001: PCR-Machine, centrifuges, EB-bath Room Nr. 1002: gel-documentation, photometer, speed vac Room Nr. 1003: laboratory, laminar-flow class 2, -20°C and 4°C refrigerator Room Nr. 1004: sterilisator, autoclave, dishwasher Room Nr. 1005: -80°C refrigerator, ultracentrifugation Room Nr. 1006: microscopy Room Nr. 1007: incubator room 37°C Room Nr. 1008: incubator room 28°C The walls, floors, and working benches are impervious to water, and resistant to acids, alkalis, solvents and disinfectants. In the laboratory there is a hand wash basin with dispensers containing soap and hand disinfectant, respectively, as well as a dipenser for paper towels. for more details see the attached maps summarized description of the work: title of the work: Cloning of genomic DNA from HIV-1 in E.coli K12, Cos7-, H9- and Jurkat- cell lines description of work/fermentation process including expected results with relevance according to biosafety issues (detailed and chronological description of the intended work): 1. Cloning of HIV-1-gag, env,tat and nef into H9 and Jurkat cell lines:
HIV- genes will be amplified by PCR, modified by adding 5’ an EcoRI and 3’ a HindIII restriction site and cloned into pAB12 (which was derived from pBR322 by inserting an modified MCS and the RSV LTR promotor from pRSVneo).
H9 and Jurkat cells will then be transformed with the resulting pAB12env, pAB12gag, pAB12tat and pAB12nef plasmids
2. Cloning of HIV-1 total genome into E. coli K12: Total genomic DNA of HIV-1 will be isolated and cloned into pUC19 (pBR322 derivate). Then E. coli K12 will be transformed with the resulting plasmid pUC19HIV
3. Cloning of HIV-1- total genome into Cos7-cells: Total genomic DNA of HIV-1 will be isolated and cloned into pBR322. Then Cos7 cells will be transformed with the resulting plasmid pBR322HIV. description of the recipient organism (e. g. genotype, biosafety level, source, citations, catalogue number ans other risk assessment relevant properties) : E. coli K-12 ATCC10798, biosafety level 1 E. coli K-12 is apathogen und a laboratory strain. Because of its auxotrophy this strain is not able to survive in the environment. COS 7 ATCC CRL-1651, biosafety level 2 H9 ATCC HTB-176, biosafety level 1 Jurkat DSMZ ACC282, biosafety level 1 description of the donor organism (e. g. genotype, biosafety level, source, citations, calogue numbers and other risk assessment relevant properties): HIV-1, biosafety level 3 description and designation of used vectors including risk assessment relevant properties (e. g. antibiotic markers, promoters, origins of replication, repressors, biosafety level, source, citations, catalouge numbers and other risk assessment relevant properties): pUC19: this vector is an derivate of the biosafety vector pBR322 and contains an ampicilin resistance, the lac promotor , the lac Z’ gene, and MCS and the pMB1 ori. For more details see the attached vector map pAB12: this vector was obtained from pBR322 by inserting the RSV LTR promoter
from pRSVneo into pBR322. It contains an ampiciline and a tetracycline resistance
the pMB1 ori and a modified MCS. As this vector is a derivate of the biosafety vector pBR322 it could be also classified into biosafety level 1. For more information see the attached vector maps. pAB12gag, pAB12env, pAB12tat and pAB12nef are derivates from pAB12 containing the respective genes from HIV-1 and should be therefore biosafety pUC19HIV contains the total HIV-1 DNA. description and designation of the material and gene products used for genetic manipulation (e. g. sequence data, accession numbers, biosafety level, source, citations and other risk assessment relevant properties): HIV-1 genes gag, env, tat and nef as well as total HIV-DNA. Although HIV-1 is classified into biosafety level 3, the use of an biological safety system (E. coli K12) and derivates of the biosafety vector pBR322 allows to classify this work into biosafety level 2 Argumentation of the risk assessment with reference to human beings, animals, plants and environment (e. g. use of an approved biological safety system, no advantage for the survival of the GMO in the envirnonment compared to the recipient, no toxicity of the gene product etc.): 1. Cloning of HIV-1-gag, env, tat and nef into H9 and Jurkat: Althoug the donor organism is classified into biosafety level 3, the use of defined and characterised genes together with the use of an approved biosafety system (pBR322 derivates, recipient organisms of biosafety level 1) results in a downgrading of the biosafety level to biosafety level 2 2. Cloning of total HIV-1 DNA into E. coli K12: Although total DNA of an biosafety level 3 organism will be cloned, the use of E. coli K12 (biosafety level 1) combined with the use of an pBR322 Derivate resembles an approved biological safety system. Therefore this part of the work will be classified into biosafety level 2 3. Cloning of total HIV-1 DNA into COS 7 cells: Since the used plasmid pBR322 does not posess an eukaryotic promoter and of the immobilization of this plasmid, but based on the fact, that the donor organism
is classified level 3 and the recipient is classified on biosafety level 2, this part of the work will be classified into biosafety level 2 Classification of the GMO into a biosafety level group and description of the intended safety precautions (e. g. compliance of international GLP-/GMP- standardsm instruction of the personal, restricted access to laboratories where GMO are handled etc.): The GMOs are classified into biosafety level 2. All work where aerosols can be produced will be done in an class 2 biosafety cabinet. All work will be done in compliance of international GLP standards. The personal working with GMO will be instructed properly. A hygiene plan is provided. Also there is restricted access to the laboratory 2.9. description of the measures for the inactivation of GMO and the waste managment: All material, glassware and cultures will be inactivated by autoclaving for 30 min. at 121° C. The benches will be routinely cleaned with appropriate disinfectants (e. g. Et- OH, Na-Hypochloride). All inactivated waste will be collected in sealed barrels and incinerated declaration of the containment level to verify the biosafety level according to Part B Z 3 of the ordinance of work with GMO in the contained use, BGBl. II Nr. 431/2002 (tick where applicable):
Einschließungsstufe 1 Einschließungsstufe 2 2.11. classification of the intended work into biosafety level (tick where applicable):
3.1. information on the rules for accident precaution (only for work in biosafety level All work will be done in biosafety level 2 equipped laboratories. Process steps where hazardous quantities of aerosols are formed will be done in class two biosafety cabinets. The personal will be instructed properly. There will be restricted access to the laboratories 4. internal enabling: Assessment of the biological safety committee (BSC): the classification of the GMO into the biosafety level mentioned at point 2.11 was affirmed (tick where applicable): minutes of the BSC are attached (tick where applicable) following information should be handled confidential according to § 105 and § 106 of the Austrian Gene Tecnology act and should therefore not become public: siganture of the CARRIER: date: 05.05.2004 signature:
Mishloach Manot for Purim -David S. Taylor “Mishloach Manot” (Hebrew) or “Shlach Manos” (Yiddish) means "sending portions" and refers to the food baskets given on Purim. Jewish custom says to give at least two different types of ready-to-eat food, to at least one person at Purim. This is due to the fact that the Hebrew word “Manot” (Esther 9:22) is in the plural form
Exelon Credit Agreements Expand Relationships with Minority and Community Banks - . Preview Mail w/ Toolbar Exelon Credit Agreements Expand Relationships with Minority and Community Banks $94 million facility from 29 banks underscores commitment to working with diverse suppliers Press Release Source: Exelon Corporation On Monday October 25, 2010, 2:21 pm EDT CHICAGO--(BUS